4R9T

L-ficolin complexed to sulphates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97240
Spacegroup name	P 32 2 1
Unit cell lengths	96.110, 96.110, 141.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.050 - 2.250
R-factor	0.18939
Rwork	0.188
R-free	0.21782
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.668
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.050	20.000	2.300
High resolution limit [Å]	2.250	10.000	2.250
Number of reflections	36406
Completeness [%]	100.0	97	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	Protein solution: 5.7 mg/ml in 145 mM NaCl, 50 mM triethanolamine-HCl, pH 7.4. Reservoir solution composed of 15% (w/v) PEG 8000, 200 mM Ca acetate and 0.1 M HEPES. Ligand soaking. Cryoprotection was achieved by adding PEG 400 to the drop just before flash-cooling the crystal in liquid nitrogen, VAPOR DIFFUSION, HANGING DROP, temperature 293K