4R9N
DeoR family transcriptional regulator from Listeria monocytogenes.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 52.818, 52.818, 172.283 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.000 - 1.869 |
| R-factor | 0.1839 |
| Rwork | 0.181 |
| R-free | 0.23120 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.831 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.000 | 50.000 | 1.900 |
| High resolution limit [Å] | 1.869 | 5.070 | 1.869 |
| Rmerge | 0.114 | 0.063 | 0.886 |
| Number of reflections | 21169 | ||
| <I/σ(I)> | 8.2 | 2.33 | |
| Completeness [%] | 99.8 | 97.7 | 100 |
| Redundancy | 9 | 7.5 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 0.2 M sodium chloride, 0.1 M sodium acetate buffer, 20% PEG-8000, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
