4R99
Crystal structure of a uricase from Bacillus fastidious
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.195, 77.415, 77.777 |
| Unit cell angles | 111.22, 90.55, 114.88 |
Refinement procedure
| Resolution | 40.288 - 1.800 |
| R-factor | 0.2293 |
| Rwork | 0.228 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r8x |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.910 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 122119 | |
| Completeness [%] | 95.2 | 95.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.1M Tris-HCl, 0.2M ammonium sulfate, 25%(w/v) PEG-3350 , pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
