4R8W

Crystal structure of H7 hemagglutinin from A/Anhui/1/2013 in complex with a neutralizing antibody CT149

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	H 3 2
Unit cell lengths	126.856, 126.856, 409.596
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.405 - 2.795
R-factor	0.265
Rwork	0.262
R-free	0.31100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 4KOL and 4DN3
RMSD bond length	0.004
RMSD bond angle	0.780
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.795	2.795
Number of reflections	31761
Completeness [%]	99.4	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	291	30% v/v PEG 4000, 60mM Divalents, pH 8.5, VAPOR DIFFUSION, temperature 291K