4R8V

Crystal structure of the hydrolase domain of 10-formyltetrahydrofolate dehydrogenase (wild-type) complex with formate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	110
Detector technology	CCD
Collection date	2014-08-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	103.980, 51.977, 59.936
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.962 - 2.197
R-factor	0.1949
Rwork	0.193
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ts4
RMSD bond length	0.007
RMSD bond angle	1.114
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.197	2.197
Number of reflections	17126
Completeness [%]	99.6	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	0.1~0.2M Bis-Tris, pH 5.5, (25~29%(w/v) PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K