4R8H
The role of protein-ligand contacts in allosteric regulation of the Escherichia coli Catabolite Activator Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Detector technology | PIXEL |
| Collection date | 2013-07-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9687 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.730, 102.260, 54.300 |
| Unit cell angles | 90.00, 111.55, 90.00 |
Refinement procedure
| Resolution | 51.130 - 1.460 |
| R-factor | 0.18858 |
| Rwork | 0.187 |
| R-free | 0.21499 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.806 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 51.130 |
| High resolution limit [Å] | 1.460 |
| Number of reflections | 80040 |
| Completeness [%] | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 3350, MPD, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
