4R83

Crystal structure of Sialyltransferase from Photobacterium damsela

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-23
Detector	MAR CCD 165 mm
Wavelength(s)	0.97946
Spacegroup name	P 1
Unit cell lengths	64.670, 81.340, 111.840
Unit cell angles	98.60, 92.76, 100.85

Refinement procedure

Resolution	34.839 - 1.930
R-factor	0.1807
Rwork	0.179
R-free	0.21920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z4t
RMSD bond length	0.007
RMSD bond angle	1.076
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.980
High resolution limit [Å]	1.930	1.930
Rmerge	0.029	0.397
Number of reflections	156324
<I/σ(I)>	18.42	2.16
Completeness [%]	94.2	94
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	20% PEG 6000, 0.2M sodium chloride, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K