4R7X

Crystal structure of N-lobe of human ARRDC3(1-180)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-27
Detector	RAYONIX MX300HS
Wavelength(s)	1.00000
Spacegroup name	P 41 21 2
Unit cell lengths	107.941, 107.941, 117.778
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.026 - 2.610
R-factor	0.2104
Rwork	0.208
R-free	0.25180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.697
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.740
High resolution limit [Å]	2.610	2.610
Number of reflections	20902
Completeness [%]	96.4	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	0.75M NH4H2PO4, 0.1M Sodium citrate tribasic dehydrate, 3.0% (W/V) 6-Aminohexanoic acid, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K