4R7V
Crystal structure of N-lobe of human ARRDC3(1-165)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11587 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 67.547, 36.158, 74.331 |
| Unit cell angles | 90.00, 109.47, 90.00 |
Refinement procedure
| Resolution | 28.829 - 1.730 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.22090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.925 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.790 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Number of reflections | 17177 | |
| Completeness [%] | 99.9 | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 20% PEG 3350, 0.1M Tris-HCl, 0.2M (NH4)2SO4, 50mM Guanidine-HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
