4R7T
Crystal structure of glucosamine-6-phosphate deaminase from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.359, 135.124, 86.001 |
| Unit cell angles | 90.00, 106.15, 90.00 |
Refinement procedure
| Resolution | 38.650 - 2.100 |
| R-factor | 0.2022 |
| Rwork | 0.199 |
| R-free | 0.25360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fs5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.002 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: dev_1750)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 47655 | |
| <I/σ(I)> | 10.3 | 1.52 |
| Completeness [%] | 94.5 | 80.7 |
| Redundancy | 2.3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2M magnesium chloride, 0.1M HEPES pH7.5, 30% PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
