4R7Q
The structure of a sensor domain of a histidine kinase from Vibrio cholerae O1 biovar eltor str. N16961
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 88.600, 88.600, 106.993 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.157 - 1.981 |
| R-factor | 0.1847 |
| Rwork | 0.184 |
| R-free | 0.20270 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.200 | 2.010 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.097 | 0.647 |
| Number of reflections | 30252 | |
| <I/σ(I)> | 49.8 | 4 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 11.5 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.8M Lithium sulfate monohydrate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
