4R7D
Fab Hu 15C1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9764 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.820, 82.510, 261.570 |
| Unit cell angles | 90.00, 101.21, 90.00 |
Refinement procedure
| Resolution | 14.999 - 2.753 |
| R-factor | 0.1779 |
| Rwork | 0.177 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.398 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.820 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 109500 | |
| <I/σ(I)> | 2.83 | |
| Completeness [%] | 98.8 | 92.9 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 291 | 20% PEG 3350, 0.1M HEPES pH 7.3, 0.2M ammonium formate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
