4R79
Mos1 transposase paired-end complex with left transposon end
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 70 |
| Detector technology | CCD |
| Collection date | 2010-10-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9765 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 119.783, 86.885, 132.379 |
| Unit cell angles | 90.00, 99.03, 90.00 |
Refinement procedure
| Resolution | 29.870 - 3.100 |
| R-factor | 0.22937 |
| Rwork | 0.227 |
| R-free | 0.27009 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hos |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.851 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.154 | 0.359 |
| Number of reflections | 48795 | |
| <I/σ(I)> | 9.9 | 2.3 |
| Completeness [%] | 99.5 | 99.1 |
| Redundancy | 5 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | 100 mM Sodium Citrate pH 5.8, 100 mM Ammonium Acetate, 450 mM KCl, and 5 mM MnCl2, hanging drop, temperature 290K, VAPOR DIFFUSION, HANGING DROP |
