4R6L
Crystal structure of bacteriophytochrome RpBphP2 from photosynthetic bacterium R. palustris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-08 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 61 |
| Unit cell lengths | 174.396, 174.396, 95.672 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.021 - 3.395 |
| R-factor | 0.2704 |
| Rwork | 0.268 |
| R-free | 0.32190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ool |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.902 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.306 |
| Rmerge | 0.057 |
| Number of reflections | 22049 |
| <I/σ(I)> | 15 |
| Completeness [%] | 62.0 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 10mg/ml protein, 12% polyacrylic acid, 0.1M MgCl2, 0.1M Hepes (pH7.0), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
