4R6J
Crystal structure of computaional designed Lucine rich repeats DLRR_H in space group P212121
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.997 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.775, 96.498, 136.345 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| R-factor | 0.22078 |
| Rwork | 0.219 |
| R-free | 0.25637 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.528 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (MR) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 27148 | |
| <I/σ(I)> | 17.2 | 3.9 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 7.2 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 22% PEG 3350, 300 mM ammonium Sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
