4R62
Structure of Rad6~Ub
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2014-08-10 |
Detector | PILATUS3 6M |
Wavelength(s) | 1.03 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.666, 58.412, 115.722 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.107 - 2.280 |
R-factor | 0.2128 |
Rwork | 0.210 |
R-free | 0.26450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1AYZ and 1UBQ |
RMSD bond length | 0.010 |
RMSD bond angle | 1.194 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.106 |
Number of reflections | 11656 |
<I/σ(I)> | 2.15 |
Completeness [%] | 96.5 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 24% PEG 300, 50 mM sodium acetate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |