4R5K
Crystal structure of the DnaK C-terminus (Dnak-SBD-B)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0750 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.636, 44.827, 63.174 |
| Unit cell angles | 77.30, 78.14, 72.96 |
Refinement procedure
| Resolution | 41.239 - 1.747 |
| R-factor | 0.1783 |
| Rwork | 0.176 |
| R-free | 0.21760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.319 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.747 | 1.747 |
| Rmerge | 0.063 | 0.492 |
| Number of reflections | 43139 | |
| <I/σ(I)> | 18.6 | 2.6 |
| Completeness [%] | 96.8 | 94.9 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.15 M potassium bromide, 30% w/v polyethylene glycol monomethyl ether 2000, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
