4R4L

Crystal structure of wt cGMP dependent protein kinase I alpha (PKGI alpha) leucine zipper

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	77
Detector technology	CCD
Collection date	2012-05-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	.977488
Spacegroup name	P 62 2 2
Unit cell lengths	95.926, 95.926, 63.805
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.921 - 2.245
R-factor	0.2576
Rwork	0.254
R-free	0.28980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.501
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000
High resolution limit [Å]	2.245
Number of reflections	8669
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	40% PEG 400, 200 mM Lithium Sulfate, 0.1 M Tris base/ Hydrochloric acid, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K