4R4F
Crystal structure of non-neutralizing, A32-like antibody 2.2c in complex with HIV-1 YU2 gp120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-12-11 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.0000 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 158.321, 171.230, 227.974 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.754 - 3.514 |
| R-factor | 0.2549 |
| Rwork | 0.251 |
| R-free | 0.29480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jzz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.106 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.523 | |
| Number of reflections | 16141 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 85.7 | 59.5 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 10% MPD, 1.5 M lithium sulfate and 100 mM Imidazole pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
