4R3H
The crystal structure of an apo RNA binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-03-05 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.264, 103.026, 41.585 |
| Unit cell angles | 90.00, 105.86, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.900 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.24000 |
| Structure solution method | MIR |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.338 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.2.17) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.310 | 27.310 | 1.840 |
| High resolution limit [Å] | 1.800 | 9.020 | 1.800 |
| Rmerge | 0.086 | 0.023 | 0.901 |
| Rpim | 0.014 | 0.574 | |
| Total number of observations | 836 | 4968 | |
| Number of reflections | 28931 | ||
| <I/σ(I)> | 10.9 | 45.5 | 1.7 |
| Completeness [%] | 99.1 | 95.7 | 90.3 |
| Redundancy | 3.6 | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 25% PEG3350, 0.2M Ammonium Sulphate, 0.1M bis-tris, pH 6.5, vapor diffusion, temperature 293K |
