4R2Z
Molecular Analysis of the PDZ4 Domain of Mouse PDZK1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2014-04-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.256, 89.214, 33.809 |
| Unit cell angles | 90.00, 113.41, 90.00 |
Refinement procedure
| Resolution | 44.610 - 1.700 |
| R-factor | 0.18867 |
| Rwork | 0.187 |
| R-free | 0.22660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.713 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 22402 | |
| Completeness [%] | 89.9 | 51.1 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 0.15M Potassium Bromide, 24% PEGMME 2000, 15% Ethylene glycol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
