4R2R
Wilms Tumor Protein (WT1) zinc fingers in complex with carboxylated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-21 |
| Detector | RAYONIX MX300HS |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 66.957, 77.518, 35.585 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.121 - 2.089 |
| R-factor | 0.1945 |
| Rwork | 0.193 |
| R-free | 0.22250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r2e |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.680 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.121 | 2.160 |
| High resolution limit [Å] | 2.089 | 2.090 |
| Rmerge | 0.079 | 0.427 |
| Number of reflections | 11476 | |
| <I/σ(I)> | 13.1 | 3.5 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 8.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 28% (w/v) PEGMME 2000, 0.1 M BisTris pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
