4R2Q
Wilms Tumor Protein (WT1) zinc fingers in complex with formylated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 67.116, 78.675, 35.705 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.447 - 1.540 |
| R-factor | 0.1621 |
| Rwork | 0.161 |
| R-free | 0.19280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r2e |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.108 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.447 | 1.600 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.078 | 0.668 |
| Number of reflections | 28341 | |
| <I/σ(I)> | 17.7 | 2.2 |
| Completeness [%] | 98.5 | 99.9 |
| Redundancy | 5.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 12% (w/v) PEG 3350, 4% (v/v) Tacsimate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
