4R2M
Crystal Structure of R134D mutant of YnaF (Universal Stress Protein F) from Salmonella typhimurium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-20 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.978565 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 131.180, 131.180, 33.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.480 - 2.200 |
R-factor | 0.19728 |
Rwork | 0.195 |
R-free | 0.23435 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 4R2L |
RMSD bond length | 0.011 |
RMSD bond angle | 1.814 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.480 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 15273 | |
<I/σ(I)> | 3.5 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.7 | 9.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Under oil, Microbatch | 8.5 | 298 | 30% PEG 4000, 100mM Tris-HCl pH 8.5, 200mM Magnesium chloride, 5mM Adenosine 5'-(beta,gamma-imido)triphosphate, Under oil, Microbatch, temperature 298.0K |