4R2L
Crystal structure of YnaF (Universal Stress Protein F) from Salmonella typhimurium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.953720 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 132.760, 132.760, 32.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.190 - 1.800 |
R-factor | 0.19133 |
Rwork | 0.189 |
R-free | 0.23471 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 3FH0 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.915 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.190 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 27850 | |
<I/σ(I)> | 3.4 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 10.2 | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Under oil, Microbatch | 8.5 | 298 | 30% PEG 4000, 100mM Tris-HCl pH 8.5, 200mM Magnesium chloride, 100mM guanidine hydrochloride, Under oil, Microbatch, temperature 298.0K |