4R2B

Crystal structure of sugar transporter Oant_3817 from Ochrobactrum anthropi, target EFI-510528, with bound glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-31
Detector	ADSC QUANTUM 315
Spacegroup name	P 1 21 1
Unit cell lengths	61.457, 61.374, 96.260
Unit cell angles	90.00, 103.18, 90.00

Refinement procedure

Resolution	36.760 - 1.870
R-factor	0.17667
Rwork	0.175
R-free	0.23070
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.201
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.900
High resolution limit [Å]	1.870	1.870
Rmerge	0.133	0.980
Number of reflections	57914
<I/σ(I)>	13	1.6
Completeness [%]	99.9	98.2
Redundancy	6	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4		PROTEIN: 10 MM BIS-TRIS, 500 MM NACL, 5% GLYCEROL, 5 MM DTT, TEV PROTEASE (1:100 RATIO). RESERVOIR: 1.0 M LITHIUM CHLORIDE, 0.1 M SODIUM CITRATE:HCL, 20% PEG6000, pH 4.0, VAPOR DIFFUSION, SITTING DROP