4R29

Crystal structure of bacterial cysteine methyltransferase effector NleE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	200
Detector technology	CCD
Collection date	2011-05-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	134.304, 53.976, 134.794
Unit cell angles	90.00, 90.11, 90.00

Refinement procedure

Resolution	20.020 - 2.310
R-factor	0.1991
Rwork	0.197
R-free	0.23380
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.108
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.6.3_473))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.020	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	42616
Completeness [%]	99.8	98.4
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1