4R27
Crystal structure of beta-glycosidase BGL167
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.498, 146.940, 53.041 |
Unit cell angles | 90.00, 101.99, 90.00 |
Refinement procedure
Resolution | 23.200 - 2.030 |
R-factor | 0.19328 |
Rwork | 0.191 |
R-free | 0.23932 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cmj |
RMSD bond length | 0.017 |
RMSD bond angle | 1.849 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.030 | 2.030 |
Number of reflections | 44261 | |
Completeness [%] | 97.2 | 98.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 291 | 36% MPD-PEG 1000-PEG 3350 mixture, 55mM calcium chloride, pH 7.5, VAPOR DIFFUSION, temperature 291K |