4R25
Structure of B. subtilis GlnK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 66.600, 66.600, 189.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.485 - 2.519 |
| R-factor | 0.217 |
| Rwork | 0.213 |
| R-free | 0.25490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z0g |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.035 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 63.100 |
| High resolution limit [Å] | 2.519 |
| Number of reflections | 5695 |
| <I/σ(I)> | 14.3 |
| Completeness [%] | 99.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 10% Peg 8000, 0.1 M MES, 0.1 M zinc acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
