4R1K

Crystal structure of a NTF2-like protein (EUBSIR_01394) from Eubacterium siraeum DSM 15702 at 2.56 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97939,0.91837,0.97882
Spacegroup name	C 1 2 1
Unit cell lengths	105.646, 59.318, 58.411
Unit cell angles	90.00, 101.38, 90.00

Refinement procedure

Resolution	42.844 - 2.560
R-factor	0.2551
Rwork	0.253
R-free	0.29890
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.483
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.844		2.650
High resolution limit [Å]	2.560	5.480	2.560
Rmerge	0.102	0.051	0.652
Number of reflections	11309	1172	1109
<I/σ(I)>	10.16	20.4	2.1
Completeness [%]	97.5	96.1	97.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.2M magnesium acetate, 30.0% 2-methyl-2,4-pentanediol, 0.1M sodium cacodylate pH 6.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K