4R1H
GntR family transcriptional regulator from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.032, 68.398, 84.016 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.700 - 1.760 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.20880 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.765 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 31.700 | 50.000 | 1.790 |
| High resolution limit [Å] | 1.760 | 4.780 | 1.760 |
| Rmerge | 0.069 | 0.043 | 0.781 |
| Number of reflections | 31502 | ||
| <I/σ(I)> | 11.4 | 2.06 | |
| Completeness [%] | 99.9 | 97.9 | 100 |
| Redundancy | 7.5 | 6.9 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 297 | 0.17 M sodium acetate, 0.085 M Tris-HCl buffer, 25.5% PEG 4000, 15% glycerol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
