4R1C
Crystal Structure of 3D7 strain Plasmodium falciparum AMA1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 156.529, 54.500, 67.955 |
Unit cell angles | 90.00, 91.42, 90.00 |
Refinement procedure
Resolution | 40.863 - 2.000 |
R-factor | 0.1961 |
Rwork | 0.193 |
R-free | 0.24730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1z40 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.075 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 41.200 |
High resolution limit [Å] | 2.000 |
Number of reflections | 38986 |
Completeness [%] | 100.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 12-15 % (v/v) polyethylene glycol 3350, 0.02 M MES and 10 mM manganese chloride. Crystals were dehydrated overnight in reservoir solution with increased (35%) (v/v) polyethylene glycol 3350 before cryo-stabilisation in 38% (v/v) polyethylene glycol 3350, 0.088 M MES (pH 6.0) and 44 mM manganese chloride for 6-8 h prior to data collection. 5% (v/v) methanol was added to the stabilisation solution, VAPOR DIFFUSION, HANGING DROP, temperature 298K |