4R16
Structure of UDP-D-MAnNAc dehdrogeanse from Pyrococcus horikoshii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-01-09 |
| Detector | RIGAKU |
| Wavelength(s) | 0.9311, 0.9721, 0.9521 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.030, 74.650, 73.560 |
| Unit cell angles | 66.20, 70.40, 75.20 |
Refinement procedure
| Resolution | 40.000 - 1.550 |
| Rwork | 0.224 |
| R-free | 0.25060 |
| Structure solution method | MAD |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 135913 | |
| Completeness [%] | 96.0 | 95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 291 | PEG 8000, 0.1M HEPES, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
