4R0N
Hexagonal form of phosphopantetheine adenylyltransferase from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 32 |
| Unit cell lengths | 106.472, 106.472, 71.323 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.640 - 2.000 |
| R-factor | 0.21264 |
| Rwork | 0.210 |
| R-free | 0.26554 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e1a |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.350 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 57597 |
| Completeness [%] | 94.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | Counter diffusion |
