4R0J
The crystal structure of a functionally uncharacterized protein SMU1763c from Streptococcus mutans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-30 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97937 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 57.816, 112.544, 83.323 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.630 - 1.715 |
| R-factor | 0.1778 |
| Rwork | 0.176 |
| R-free | 0.20600 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.029 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.630 | 1.750 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.087 | 0.689 |
| Number of reflections | 29428 | |
| <I/σ(I)> | 46.4 | 2.92 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.3 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.1M Tris, 1.5M Ammonium sulfide, 12% Glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
