4QZU

Crystal Structure of wild type Human Cellular Retinol Binding Protein II (hCRBPII) bound to retinol at 11 KeV beam energy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-20
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 1
Unit cell lengths	36.476, 54.139, 68.298
Unit cell angles	107.64, 96.94, 103.71

Refinement procedure

Resolution	34.660 - 1.500
R-factor	0.165
Rwork	0.162
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rct
RMSD bond length	0.007
RMSD bond angle	1.066
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.660	1.510
High resolution limit [Å]	1.496	1.500
Rmerge	0.042	0.598
Number of reflections	76284
<I/σ(I)>	26.45	2.41
Completeness [%]	95.2	88
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5	298	25% PEG 4000 (40%), 0.1M Sodium Acetate pH 4.5, Ammonium Acetate 0.1M, VAPOR DIFFUSION, HANGING DROP, temperature 298K