4QZD
Mouse Tdt, F405A mutant, in complex with a DSB substrate, C-C base pair
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.915010 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 55.933, 74.920, 125.633 |
Unit cell angles | 90.00, 98.43, 90.00 |
Refinement procedure
Resolution | 43.560 - 2.700 |
R-factor | 0.2019 |
Rwork | 0.200 |
R-free | 0.24520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4i2a |
RMSD bond length | 0.010 |
RMSD bond angle | 0.960 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.069 | 0.830 |
Number of reflections | 14140 | |
<I/σ(I)> | 15.3 | 2 |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 5.6 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 12-17% PEG4000, 9-12% isopropanol, 100 mM sodium acetate, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |