4QZB
Mouse Tdt in complex with a DSB substrate, C-T base pair
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.919600 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 57.110, 75.200, 127.620 |
| Unit cell angles | 90.00, 99.58, 90.00 |
Refinement procedure
| Resolution | 26.550 - 2.150 |
| R-factor | 0.1886 |
| Rwork | 0.187 |
| R-free | 0.21970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4i2a |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.049 | 0.608 |
| Number of reflections | 28987 | |
| <I/σ(I)> | 14.3 | 2.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 12-17% PEG4000, 9-12% isopropanol, 100 mM sodium acetate, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
