4QZ9
Mouse Tdt in complex with a DSB substrate, C-A base pair
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.919600 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 56.936, 74.720, 126.357 |
| Unit cell angles | 90.00, 100.06, 90.00 |
Refinement procedure
| Resolution | 39.060 - 2.050 |
| R-factor | 0.1846 |
| Rwork | 0.183 |
| R-free | 0.20430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4i2a |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.040 | 0.503 |
| Number of reflections | 32370 | |
| <I/σ(I)> | 15.7 | 2.5 |
| Completeness [%] | 98.9 | 97.8 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 12-17% PEG4000, 9-12% isopropanol, 100 mM sodium acetate, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
