4QY8
Crystal Structure of anti-MSP2 Fv fragment (mAb6D8) in complex with 3D7-MSP2 14-30
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-10 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.107, 60.323, 43.477 |
| Unit cell angles | 90.00, 107.03, 90.00 |
Refinement procedure
| Resolution | 34.230 - 1.353 |
| R-factor | 0.1525 |
| Rwork | 0.152 |
| R-free | 0.16470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qyo |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.390 | 1.430 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.590 | |
| Number of reflections | 44204 | |
| <I/σ(I)> | 2.1 | |
| Completeness [%] | 97.4 | 95.8 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 30% PEG 8000, 0.2M NaCl, 0.1M NaOAc, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
