4QY7

Crystal structure of a yobA protein (BSU18810) from Bacillus subtilis subsp. subtilis str. 168 at 1.55 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-11
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.95369,0.97937,0.97922
Spacegroup name	P 21 21 21
Unit cell lengths	27.556, 37.988, 81.245
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.746 - 1.550
R-factor	0.1918
Rwork	0.190
R-free	0.22320
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.080
Data reduction software	XDS
Data scaling software	XSCALE (January 10, 2014 BUILT=20140307)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.746	27.746	1.610
High resolution limit [Å]	1.550	3.330	1.550
Rmerge	0.031	0.016	0.521
Number of reflections	12907	2346	2442
<I/σ(I)>	14.83	42.8	1.6
Completeness [%]	98.3	96.4	94.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	5.00% polyethylene glycol 3000, 40.00% polyethylene glycol 400, 0.1M MES pH 6.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K