4QY5
Crystal structures of chimeric beta-lactamase cTEM-19m showing different conformations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-12 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.520, 58.210, 109.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.400 - 1.501 |
| R-factor | 0.11283 |
| Rwork | 0.110 |
| R-free | 0.16101 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zg4 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.759 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.400 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.062 | 0.841 |
| Number of reflections | 38195 | |
| <I/σ(I)> | 20.9 | 2.2 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 7.2 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 30% PEG 4000, 0.2M magnesium chloride, 0.1M TrisHCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
