4QVG

Crystal structure of S-adenosylmethionine-dependent methyltransferase SibL in its apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	200
Detector technology	CCD
Collection date	2013-06-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	F 2 2 2
Unit cell lengths	102.931, 295.421, 322.231
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.116 - 2.900
R-factor	0.2135
Rwork	0.211
R-free	0.26580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4u1q
RMSD bond length	0.010
RMSD bond angle	1.382
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge		0.211
Number of reflections	54730
Completeness [%]	99.7	100
Redundancy		5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	277	10%(v/v) Polyethylene glycol 200, 0.1 M BIS-TRIS propane pH 9.0, 18%(w/v) Polyethylene glycol 8,000 , VAPOR DIFFUSION, SITTING DROP, temperature 277K