4QUV
Structure of an integral membrane delta(14)-sterol reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 |
| Unit cell lengths | 74.660, 74.615, 79.549 |
| Unit cell angles | 66.00, 90.37, 86.86 |
Refinement procedure
| Resolution | 37.255 - 2.743 |
| R-factor | 0.2355 |
| Rwork | 0.233 |
| R-free | 0.28370 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.646 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.250 | 2.840 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.041 | 0.415 |
| Number of reflections | 30541 | |
| <I/σ(I)> | 21.1 | 1.4 |
| Completeness [%] | 74.8 | 28.5 |
| Redundancy | 2.1 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M Tris, 0.2 M NH4Ac, 30% (v/v) Pentaerythritol ethoxylate (15/4 EO/OH), pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
