4QTQ
Structure of a Xanthomonas Type IV Secretion System related protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-25 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54187 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.457, 37.695, 43.252 |
| Unit cell angles | 90.00, 92.61, 90.00 |
Refinement procedure
| Resolution | 38.780 - 2.000 |
| R-factor | 0.20224 |
| Rwork | 0.200 |
| R-free | 0.24072 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.186 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.780 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.065 | 0.497 |
| Number of reflections | 16066 | |
| <I/σ(I)> | 8.8 | 2.1 |
| Completeness [%] | 95.1 | 89.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 100 mM BIS-TRIS-HCL PH 5.5, 200 mM AMMONIUM SULPHATE AND 25% (W/V) PEG 3350, VAPOR DIFFUSION , VAPOR DIFFUSION, SITTING DROP, temperature 291K |
