4QSZ
Crystal structure of mouse JMJd7 fused with maltose-binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-28 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.090, 158.920, 100.010 |
| Unit cell angles | 90.00, 94.08, 90.00 |
Refinement procedure
| Resolution | 62.153 - 2.860 |
| R-factor | 0.1948 |
| Rwork | 0.191 |
| R-free | 0.26410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.383 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.680 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Number of reflections | 44978 | |
| Completeness [%] | 96.0 | 91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8 | 277 | Hampton Research PEG/ION HT (89): 20 mM sodium citrate, 80 mM Bis-Tris propane, 16% PEG3350, pH 8.0, EVAPORATION, temperature 277K |
