4QSC

Crystal structure of ATU4361 sugar transporter from Agrobacterium Fabrum C58, target efi-510558, with bound maltose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-20
Detector	PSI PILATUS 6M
Spacegroup name	P 1 21 1
Unit cell lengths	36.122, 76.279, 68.169
Unit cell angles	90.00, 90.66, 90.00

Refinement procedure

Resolution	50.000 - 1.300
R-factor	0.13891
Rwork	0.138
R-free	0.17433
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.676
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.320
High resolution limit [Å]	1.300	1.300
Rmerge	0.067	0.450
Number of reflections	83480
<I/σ(I)>	19.4	2.7
Completeness [%]	92.1	62.6
Redundancy	4.3	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8		;2.10 CRYSTALLIZATION CONDITIONS, PROTEIN: 10 MM BIS-TRIS, 500 MM NACL, 10% GLYCEROL, 5 MM DTT, TEV PROTEASE (1:100 RATIO); RESERVOIR: 0.1M MES-NAOH, PH 6.5, 20% PEG400; CRYOPROTECTION: RESERVOIR PLUS 100MM MALTOSE, VAPOR DIFFUSION, SITTING DROP