4QRZ

Crystal structure of sugar transporter atu4361 from agrobacterium fabrum c58, target efi-510558, with bound maltotriose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-20
Detector	PSI PILATUS 6M
Spacegroup name	P 1 21 1
Unit cell lengths	36.554, 77.064, 66.143
Unit cell angles	90.00, 93.22, 90.00

Refinement procedure

Resolution	50.000 - 1.340
R-factor	0.11245
Rwork	0.111
R-free	0.14704
Structure solution method	SAD
RMSD bond length	0.020
RMSD bond angle	1.800
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.360
High resolution limit [Å]	1.340	1.340
Rmerge	0.037	0.191
Number of reflections	80995
<I/σ(I)>	27.5	6.4
Completeness [%]	98.7	100
Redundancy	7.3	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5		PROTEIN: 10 MM BIS-TRIS, 500 MM NACL, 10% GLYCEROL, 5 MM DTT, TEV PROTEASE (1:100 RATIO); RESERVOIR: 0.1 M SODIUM ACETATE, PH 4.5, 20% PEG3350; CRYOPROTECTION: RESERVOIR; VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K, VAPOR DIFFUSION, HANGING DROP