4QR0
Crystal structure of Streptococcus pyogenes Cas2 at pH 5.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-20 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 54.803, 54.803, 124.013 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.000 - 2.400 |
| R-factor | 0.19863 |
| Rwork | 0.196 |
| R-free | 0.24623 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4es2 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.637 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.624 | |
| Number of reflections | 7806 | |
| <I/σ(I)> | 20.51 | 4.43 |
| Completeness [%] | 98.1 | 97.8 |
| Redundancy | 13.6 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 30% (w/v) PEG 4000, 200mM ammonium acetate, 100mM tri-sodium citrate pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
