4QQX
Crystal structure of T. fusca Cas3-ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 87.250, 222.807, 125.087 |
Unit cell angles | 90.00, 104.10, 90.00 |
Refinement procedure
Resolution | 46.980 - 3.340 |
R-factor | 0.1733 |
Rwork | 0.172 |
R-free | 0.22660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qqw |
RMSD bond length | 0.015 |
RMSD bond angle | 1.561 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.490 |
High resolution limit [Å] | 3.340 | 3.340 |
Rmerge | 0.064 | 0.412 |
Number of reflections | 66466 | |
<I/σ(I)> | 9.3 | 2.6 |
Completeness [%] | 99.2 | 99.6 |
Redundancy | 2.2 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 100 mM MES, pH 6.5, and 5% 10% (w/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 100 mM MES, pH 6.5, and 5% 10% (w/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K |